Unlimited DNA from a few cells.

The
growing trend to study disease and drug response at the molecular level
has focused attention on DNA as a precious resource. Multiple Displacement
Amplification (MDA) technology developed by MSI enables the first effective
whole genome amplification method. The MDA method is a quantum leap over
other currently available whole genome amplification methods, which are
based on PCR
The MSI MDA method rapidly amplifies the genome with comprehensive loci
coverage and minimal bias between any loci, yielding 10+kb fragments in
a simple, scalable reaction.

The MSI MDA technology platform for whole genome amplification can transform
the current practice of sample preparation and sample archiving for genetic
testing.

• Comprehensive coverage and 10+kb fragments expand applications
• Increased accuracy provides amplified material true to the original
• Simplified method eliminates sample preparation step
• Provides assay-ready DNA
  

  

  The MDA technology platform for whole genome
  amplification enables:

  • A virtually unlimited number of tests per sample
  • Use of smaller samples, such as buccal swabs, mouthwash, and finger prick
  • Use of DNA from alternative sources, such as biopsies and needle aspirates
  • An increase in the practice of archiving DNA for additional testing or re-testing
  • Amplification directly from whole blood and cells

  Optimal coverage and minimal bias
  

  Comprehensive coverage of all gene sequences and unprecedented low bias expand range of downstream applications.
  

  Accuracy
  

  Use of enzyme with very high proofreading capability yields amplified DNA true to original sequence, enabling high-performance assays such as SNP, genotyping.

  Scalability
  

  Larger quantities of DNA generated by increasing reaction volume, enables large-scale studies such as high-density genotyping.

  Sample prep steps eliminated

  MDA method is carried out directly on crude lysed cells, whole blood, buccal swabs, and other biological samples, eliminating costly, time-consuming DNA purification steps.

  Assay-ready
  

  Contaminants effectively diluted out, replacing need for DNA purification and allowing downstream applications to be performed with no inhibitory effects.

  High throughput
  

  Ideally suited for 96-well-plate automated systems with no centrifugation and/or filtrations needed.

  Long fragments

  DNA generated in excess of 10kb in length, enabling molecular biology methods such as RFLP.

  VITAL
  CHARACTERISTICS:

  Sample Targets	Genomic DNA, whole blood, buffy coats, buccal swabs, mouthwash, and cultured cells
  Amount	1µl blood, single cheek swab, sub-ng gDNA
  Lysis Procedures	10-minute one-step lysis
  Yield	50µg DNA from a few genomic copies in a 100µl reaction Scalable to 10mg DNA yield from large-scale reaction Consistent yield independent of amount of starting sample
  Reaction Time	6 hours or overnight
  Coverage	All loci represented at approximately same level as in starting genomic DNA target, no more than 6-fold difference Only highly repetitive sequences lost, such as centromere and telomere repeats
  Assay-ready DNA	PCR-based assays, such as SBE, TaqMan, restriction enzyme digestion, and STRs Southern analysis, including RFLP DNA sequencing

  TaqMan is a registered trademark of Roche Molecular
  Systems