Molecular Staging

The specificity of the DNA segments catalyzed by the enzyme DNA ligase can be exploited in systems designed to identify the presence of a target sequence of nucleic acids in a biological sample. The amplification methods based on the ligase can...

The basic principle of any molecular diagnostic technology is the detection of a specific sequence of nucleic acids by hybridization with a complementary sequence, the probe, followed by the detection of the hybrid. However, the sensitivity of tests using...

The introduction of rapid methods of sequencing in the late '70s represented a major shift in studies of two methods of DNA sequencing of nucleic acids. One of them (Maxam and Gilbert) chemical reagents used to cut DNA at specific base. The other is...