Southern blot (SB). The Commonly Used probes are Homologous to IR1, as this sequence is Repeat 10 times, Resulting in increaser sensitivity of detection. SB has Some Advantages in the detection of EBV tissue:


It is the only system That Could certify the monoclonality of EBV infection. The Presence of a single fused terminal band Suggests That the infected cells represent clonal expansion of a single cell with latent viral infection. In contrast to the Presence of multiple bands That Reflect episomes with variable numbers of TRs, characteristic of the Existence of Multiple Infections and, Therefore, poly-or oligoclonal Infections (8).


SB analysis of the IRs to Recognize the lytic infection. The linear EBV DNA digestion with BamHI releases a terminal fragment (right) of 4 kb fused with a variable number of IRs and terminal fragment (LHS) of 3.5 kb fused with a variable number of IRs (9).


In Some Cases You Can determine Whether or not the SB EBV DNA in the cell genome.


In situ hybridization (ISH). It is Used with IR1 probes in frozen Homologous Tissues, fixed in cell lines and extensions of Metaphase chromosomes (10). The major disadvantage of ISH with DNA probes is low sensitivity and Their Inability to detect Therefore Their latent infections. The situation is very Different When using complementary RNA probes EBER1 and EBER2, Which are present in high numbers of copies of latent infection. Improve sensitivity PNA probes (11).


Chain reaction (PCR). His great sensitivity Profound has caused a change in the detection of AD N EBV.


Immunohistochemical techniques. With antibodies: LMP-1, EBNA-2 and ZEBRA.