Conclusion
In: Bioscience
The specificity of the DNA segments catalyzed by the enzyme DNA ligase can be exploited in systems designed to identify the presence of a target sequence of nucleic acids in a biological sample.
The amplification methods based on the ligase can increase sensitivity without causing a decrease in specificity. Have described modifications of these techniques that reduce non-specific ligation. The automation of testing in the future allow the routine use of such technology in the clinical laboratory.